Abstract

We demonstrate an all-fiber negative axicon probe with a Bessel beam for low coherence phase microscopy including refractive index measurement of a cellular level sample in reflection mode. The negative axicon chemically incised at the distal end of the optical fiber spawns the Bessel beam. The system provides a phase sensitivity of ∼0.28 mrad and optical path length sensitivity of ∼23 pm in air. The lateral resolution and working distance are found to be ∼3.91 µm and 650 µm to exhibit the performance of the system experimentally. The three-dimensional (3D) phase map of the cheek cell along with the refractive index is obtained from the reflected power spectrum. The combined low coherence phase microscopy and refractive index measurement provides the system with a potential for biological application. Also, the all-fiber probe can be easily integrated as an endoscopic probe.

© 2022 Optica Publishing Group

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