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Quantitative phase imaging by single-shot Hilbert–Huang phase microscopy

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Abstract

We propose a novel single-shot Hilbert–Huang transform-based algorithm applied to digital holographic microscopy (DHM) for robust, fast, and accurate single-shot quantitative phase imaging in on-axis and off-axis configurations. Fringe pattern with possible defects and closed fringes are adaptively filtered and accurately phase demodulated using local fringe direction estimation. Experimental validation of the proposed techniques is presented as the DHM study of microbeads and red blood cells phase samples. Obtained results compare very favorably with the Fourier approach (off-axis) and temporal phase shifting (on-axis).

© 2016 Optical Society of America

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Supplementary Material (2)

NameDescription
Visualization 1: AVI (831 KB)      Dynamic experiment with flowing micro-beads and 20× lens. (a)-(b) and (c)-(d) are the wrapped and 3D plot phase distributions retrieved using S2H2PM and FT techniques, respectively.
Visualization 2: AVI (1025 KB)      Dynamic experiment: cross-sections.

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