Abstract

The spectroscopic imaging capability of photoacoustics (PA) without the depth limitations of optical methods offers a major advantage in preclinical and clinical applications. Consecutive PA measurements with properly chosen wavelengths allow composition related information about blood or tissue. In this work, we propose and experimentally introduce modulation waveform engineering through the use of mismatched (uncorrelated or weakly correlated) linear frequency modulated signals for PA characterization and imaging. The feasibility of the method was tested on oxygen saturated hemoglobin and deoxygenated hemoglobin in vitro in a blood circulating rig. The method was also employed for in vivo imaging of a neck carcinoma tumor grown in a mouse thigh. The proposed method can increase the accuracy and speed of functional imaging by simultaneous PA probing with two wavelengths using portable laser-diode based PA imaging systems.

© 2015 Optical Society of America

Wavelength-modulated differential photoacoustic radar imager (WM-DPARI): accurate monitoring of absolute hemoglobin oxygen saturation

Sung Soo Sean Choi, Bahman Lashkari, Edem Dovlo, and Andreas Mandelis
Biomed. Opt. Express 7(7) 2586-2596 (2016)

Sensing of triglyceride concentration in blood solution using photoacoustic microscopy

Nan Wan, Zhe Li, Myeongsu Seong, Ke Zhang, Wei Niu, Rong Wu, and Sung-Liang Chen
Opt. Lett. 48(14) 3769-3772 (2023)

Simultaneous photoacoustic microscopy of microvascular anatomy, oxygen saturation, and blood flow

Bo Ning, Matthew J. Kennedy, Adam J. Dixon, Naidi Sun, Rui Cao, Brian T. Soetikno, Ruimin Chen, Qifa Zhou, K. Kirk Shung, John A. Hossack, and Song Hu
Opt. Lett. 40(6) 910-913 (2015)

Snap-shot multispectral imaging of vascular dynamics in a mouse window-chamber model

Hansford C. Hendargo, Yulin Zhao, Taylor Allenby, and Gregory M. Palmer
Opt. Lett. 40(14) 3292-3295 (2015)

Simultaneous photoacoustic imaging of intravascular and tissue oxygenation

Maomao Chen, Hailey J. Knox, Yuqi Tang, Wei Liu, Liming Nie, Jefferson Chan, and Junjie Yao
Opt. Lett. 44(15) 3773-3776 (2019)