Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Whole-body live mouse imaging by hybrid reflection-mode ultrasound and optoacoustic tomography

Not Accessible

Your library or personal account may give you access

Abstract

We present a hybrid preclinical imaging scanner that optimally supports image acquisition in both reflection-mode ultrasonography and optoacoustic (OA) tomography modes. The system comprises a quasi-full-ring tomographic geometry capable of the simultaneous dual-mode imaging through entire cross sections of mice with in-plane spatial resolution in the range of 150 and 350 μm in the respective OA and ultrasound (US) imaging modes with an imaging speed of up to 10 two-dimensional frames per second. Three-dimensional whole-body data is subsequently rendered by rapid scanning of the imaged plane. The system further incorporates rapid laser wavelength tuning for real-time acquisition of multispectral OA data, which enables studies of longitudinal dynamics as well as fast kinetics and biodistribution of contrast agents. In vivo imaging performance is demonstrated by label-free hybrid anatomical scans through living mice, as well as real-time visualization of optical contrast agent perfusion. By setting new standards for whole-body tomographic imaging performance in both the OA and pulse-echo US modes, the developed hybrid imaging approach is expected to benefit numerous applications where the availability of high-quality structural information provided by the tomographic reflection-mode US can ease interpretation of the functional and molecular imaging results attained by the OA modality.

© 2015 Optical Society of America

Full Article  |  PDF Article
More Like This
Hybrid spherical array for combined volumetric optoacoustic and B-mode ultrasound imaging

Hsiao-Chun Amy Lin, Xosé Luís Deán-Ben, Ali Ozbek, Yi-Hsuan Shao, Berkan Lafci, and Daniel Razansky
Opt. Lett. 49(6) 1469-1472 (2024)

In vivo whole-body optoacoustic scanner with real-time volumetric imaging capacity

Thomas Felix Fehm, Xosé Luís Deán-Ben, Steven James Ford, and Daniel Razansky
Optica 3(11) 1153-1159 (2016)

Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging

Rui Ma, Adrian Taruttis, Vasilis Ntziachristos, and Daniel Razansky
Opt. Express 17(24) 21414-21426 (2009)

Supplementary Material (2)

NameDescription
Visualization 1: MP4 (3655 KB)      Video shows the fly-through the stack of ultrasound and optoacoustic single-wavelength images of a mouse acquired in-vivo.
Visualization 2: MP4 (2192 KB)      Imaging of longitudinal uptake of IRDye-800CW by the bladder.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved