Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects

Not Accessible

Your library or personal account may give you access

Abstract

We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2μm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7nm in x, 2.1nm in y, and 5.7nm in z.

© 2011 Optical Society of America

Full Article  |  PDF Article
More Like This
Towards optimal point spread function design for resolving closely spaced emitters in three dimensions

James M. Jusuf and Matthew D. Lew
Opt. Express 30(20) 37154-37174 (2022)

Nanoscale three-dimensional single particle tracking by light-sheet-based double-helix point spread function microscopy

Bin Yu, Jie Yu, Weihai Li, Bo Cao, Heng Li, Danni Chen, and Hanben Niu
Appl. Opt. 55(3) 449-453 (2016)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved