Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Enhanced detection of fluorescent nanospheres using two-channel radially polarized surface plasmon microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We detected single dye-stained latex nanospheres as small as 20nm using a two-detection channel modified surface plasmon microscope. We found that a radially polarized incident beam leading to excitation of well-focused surface plasmons induces both fluorescence and elastic linear scattering from the spheres. The two complementary emitted signals were detected in parallel by the two separated channels, leading to well-colocalized images. We obtained high spatial resolutions for both channels down to 250nm in the lateral direction and 300nm along the longitudinal axis. We believe this multimodal microscope can be useful to track nano objects and to compensate for intermittent fluorescence, thanks to a permanently activated parallel scattering detection channel.

© 2010 Optical Society of America

Full Article  |  PDF Article
More Like This
Radial polarization induced surface plasmon virtual probe for two-photon fluorescence microscopy

K. J. Moh, X.-C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao
Opt. Lett. 34(7) 971-973 (2009)

Surface plasmon-enhanced and quenched two-photon excited fluorescence

Chun-Yu Lin, Kuo-Chih Chiu, Chia-Yuan Chang, Shih-Hui Chang, Tzung-Fang Guo, and Shean-Jen Chen
Opt. Express 18(12) 12807-12817 (2010)

Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy

Ruei-Yu He, Chun-Yu Lin, Yuan-Deng Su, Kuo-Chih Chiu, Nan-Shan Chang, Hua-Lin Wu, and Shean-Jen Chen
Opt. Express 18(4) 3649-3659 (2010)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved