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Simultaneous SLO/OCT imaging of the human retina with axial eye motion correction

Michael Pircher, Bernhard Baumann, Erich Götzinger, Harald Sattmann, and Christoph K. Hitzenberger

Open Access Open Access

Abstract

It has been shown that transversal scanning (or en-face) optical coherence tomography (TS-OCT) represents an imaging modality capable to record high isotropic resolution images of the human retina in vivo. However, axial eye motion still remains a challenging problem of this technique. In this paper we introduce a novel method to compensate for this eye motion. An auxiliary spectral domain partial coherence interferometer (SD-PCI) was integrated into an existing TS-OCT system and used to measure accurately the position of the cornea. A light source emitting at 1310nm was used in the additional interferometer which enabled a nearly loss free coupling of the two measurement beams via a dichroic mirror. The recorded corneal position was used to drive an additional voice coil translation stage in the reference arm of the TS-OCT system to correct for axial eye motion. Currently, the correction can be performed with an update rate of ~200Hz. The TS-OCT instrument is operated with a line scan rate of 4000 transversal lines per second which enables simultaneous SLO/OCT imaging at a frame rate of 40fps. 3D data of the human retina with isotropic high resolution, that was sufficient to visualize the human cone mosaic in vivo, is presented.

©2007 Optical Society of America

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Supplementary Material (2)

Media 1: MOV (2736 KB)     
Media 2: MOV (2967 KB)     

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Figures (7)
Fig. 1.
Fig. 1. Sketch of the experimental setup. L1 fixed lens, L2 lens mounted on a translation stage, DM dichroic mirror, RM reference mirror, TS translation stage, VC voice coil translation stage, LS light source, AOM acousto optic modulator, P polarizer, BS non polarizing beam splitter

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Fig. 2.
Fig. 2. Performance of axial eye motion correction. a) Black…recorded corneal position, red…motor position b) difference between curves in a)

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Fig. 3.
Fig. 3. Frame No. 8 of a sequence of en-face images recorded at fixed position of the translation stage. Left side: without depth correction. Right side: correction turned on. Field of view: 0.8°×0.8°. (To reduce file size, the pixel extension of the original movie was reduced by 70%; The bottom horizontal line is an artifact caused by the turning of the y-scanner.) [Media 1]

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Fig. 4.
Fig. 4. High resolution B-scan with axial motion correction turned on. (Extension: 0.8°×670µm (optical)). ILM inner limiting membrane, RNFL retinal nerve fiber layer, GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL/HFL outer nuclear layer/Henle fiber layer, ELM external limiting membrane, IS/OS junction between inner and outer segments of photoreceptors, ETPR end tips of photoreceptors, RPE retinal pigment epithelium

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Fig. 5.
Fig. 5. Frame No. 39 of a movie showing 3D data of the retina recorded with the instrument. Left side shows SLO images, the center shows en-face OCT images, right side shows extracted OCT B-scans. The white line shows the position of the corresponding B-scan (in the center image) and position of the corresponding en-face image (on the right hand side image), respectively. SLO images are displayed on a linear scale, OCT images are displayed on a logarithmic scale. Field of view of SLO and en-face OCT images: 0.8°×0.8°. Dimension of OCT B-scan: 0.8°×1mm (To reduce file size, the pixel extension of the original movie was reduced by 70%) [Media 2]

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Fig. 6.
Fig. 6. Comparison of the cone photoreceptor mosaic imaged with different imaging modalities. a) SLO image (averaged over 10 frames), b) OCT image (averaged over an optical depth of ~80µm), c) OCT retrieved from the ETPR layer, d) OCT retrieved from the IS/OS junction. Field of view of each image: 0.8°×0.8°. (For a better comparison with the SLO images all OCT images are squared and displayed on a linear scale)

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Fig. 7.
Fig. 7. Imaging planes at different depths extracted from the OCT data. a) ILM, b) RNFL, c) GCL (maximum intensity within 4 frames), d) INL (maximum intensity within 7 frames) Field of view of each image: 0.8°×0.8°. (All images are displayed on a linear scale.)

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