Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Multifluorophore localization as a percolation problem: limits to density and precision

Not Accessible

Your library or personal account may give you access

Abstract

We show that the maximum desirable density of activated fluorophores in a superresolution experiment can be determined by treating the overlapping point spread functions as a problem in percolation theory. We derive a bound on the density of activated fluorophores, taking into account the desired localization accuracy and precision, as well as the number of photons emitted. Our bound on density is close to that reported in experimental work, suggesting that further increases in the density of imaged fluorophores will come at the expense of localization accuracy and precision.

© 2016 Optical Society of America

Full Article  |  PDF Article
More Like This
3D localization of high particle density images using sparse recovery

Saiwen Zhang, Danni Chen, and Hanben Niu
Appl. Opt. 54(26) 7859-7864 (2015)

Phase stretch transform for super-resolution localization microscopy

Tali Ilovitsh, Bahram Jalali, Mohammad H. Asghari, and Zeev Zalevsky
Biomed. Opt. Express 7(10) 4198-4209 (2016)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (19)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved