Abstract

Classification, discrimination, and biochemical assignment of vibrational spectra of pollen samples belonging to 43 different species of the order Pinales has been made using three different vibrational techniques. The comparative study of transmission (KBr pellet) and attenuated total reflection (ATR) Fourier transform infrared (FT-IR) and FT-Raman spectroscopies was based on substantial variability of pollen grain size, shape, and relative biochemical composition. Depending on the penetration depth of the probe light, vibrational techniques acquire predominant information either on pollen grain walls (FT-Raman and ATR-FT-IR) or intracellular material (transmission FT-IR). Compared with the other two methods, transmission FT-IR obtains more comprehensive information and as a result achieves superior spectral identification and discrimination of pollen. The results strongly indicate that biochemical similarities of pollen grains belonging to the same plant genus or family lead to similar features in corresponding vibrational spectra. The exploitation of that property in aerobiological monitoring was demonstrated by simple and rapid pollen identification based on relatively small spectral libraries, with the same (or better) taxonomic resolution as that provided by optical microscopy. Therefore, the clear correlation between vibrational spectra and pollen grain morphology, biochemistry, and taxonomy is obtained, while successful pollen identification illustrates the practicability of such an approach in environmental studies.

Single-pollen analysis by laser-induced breakdown spectroscopy and Raman microscopy

Ana R. Boyain-Goitia, David C. S. Beddows, Ben C. Griffiths, and Helmut H. Telle
Appl. Opt. 42(30) 6119-6132 (2003)

Laser-induced breakdown spectroscopy of bacterial spores, molds, pollens, and protein: initial studies of discrimination potential

Alan C. Samuels, Frank C. DeLucia, Kevin L. McNesby, and Andrzej W. Miziolek
Appl. Opt. 42(30) 6205-6209 (2003)

Simulation and analysis of pollen coronas

Eberhard Tränkle and Bernd Mielke
Appl. Opt. 33(21) 4552-4562 (1994)

Vertical elliptical coronas caused by pollen

Pekka Parviainen, Craig F. Bohren, and Veikko Mäkelä
Appl. Opt. 33(21) 4548-4551 (1994)

Experimental simulations of pollen coronas

Werner B. Schneider and Michael Vollmer
Appl. Opt. 44(27) 5746-5753 (2005)