Abstract

A new on-column excitation and detection geometry for multiplexed array capillary electrophoresis is evaluated. The detection limit is improved 100-fold for the same laser power because of more efficient coupling of the laser beam. The illumination is also more uniform over the entire array. Stray light and cross-talk are effectively suppressed by immersing the capillaries in water to roughly match the refractive index. A charge-coupled-device (CCD) camera combined with cutoff filters provides adequate sensitivity and speed for high-speed DNA sequencing based on sieving in a polymer-matrix solution.

Effects of fluorescence excitation geometry on the accuracy of DNA fragment sizing by flow cytometry

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Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

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Light-emitting-diode-induced fluorescence detection of fluorescent dyes for capillary electrophoresis microchip with cross-polarization method

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Terahertz-capillary electrophoresis (THz-CE) for direct detection of separated substances in solutions

Keiko Kitagishi, Takayuki Kawai, Masayoshi Tonouchi, and Kazunori Serita
Opt. Mater. Express 14(2) 472-482 (2024)