Abstract

We have used the technique of laser fluorometry for chlorophyll <i>a</i> and pheophytin <i>a</i> measurements in 90% acetone/water extracts of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic of a pulsed Nd:YAG laser (λ = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process of individual zooplankton samples.

Photo-physiological variability in phytoplankton chlorophyll fluorescence and assessment of chlorophyll concentration

Alexander Chekalyuk and Mark Hafez
Opt. Express 19(23) 22643-22658 (2011)

Radiance-ratio algorithm wavelengths for remote oceanic chlorophyll determination

Frank E. Hoge, C. Wayne Wright, and Robert N. Swift
Appl. Opt. 26(11) 2082-2094 (1987)

Reducing variability that is due to secondary pigments in the retrieval of chlorophyll a concentration from marine reflectance: a case study in the western equatorial Pacific Ocean

Lydwine Gross, Robert Frouin, Cécile Dupouy, Jean Michel André, and Sylvie Thiria
Appl. Opt. 43(20) 4041-4054 (2004)

Variability in the relationship between light scattering and chlorophyll a concentration in oligotrophic tropical regions of the Western Pacific Ocean

Yanxia Liu, Yunwei Li, Liwen Yan, Zehua Zhang, Haibo Bi, and Haijun Huang
Opt. Express 32(7) 12141-12159 (2024)

Effects of a nonuniform vertical profile of chlorophyll concentration on remote-sensing reflectance of the ocean

Malgorzata Stramska and Dariusz Stramski
Appl. Opt. 44(9) 1735-1747 (2005)