Abstract

This paper presents the development of a biosensor based on optical fiber, using a polyclonal antibody kisspeptin receptor as a biological recognition element that is connected to puberty onset and may also help to suppress metastasis in melanoma breast cancer. The fiber surface was chemically prepared to immobilize the antibody. The structural homogeneity of the biosensor, at each stage of the self-assembly, was characterized by Fourier transform infrared spectroscopy and by measurements of the transmission at the output of the biosensor. The morphological homogeneity analysis was performed by optical microscopy and scanning electron microscopy. The biosensor developed was checked to detect kisspeptin in brain tissues by spectral transmission using a superluminescent diode. The data were analyzed using principal component analysis. The interaction of the kisspeptin with its counterpart by means of the evolution of the transmission spectrum as a function of time was observed.

© 2020 Optical Society of America

Optics theory and practice in Iberoamerica: introduction to the feature issue

Gustavo Olague, Sambit Bakshi, Josué Álvarez-Borrego, Joseph N. Mait, Amalia Martínez-García, and Markus E. Testorf
Appl. Opt. 59(13) IBO1-IBO5 (2020)

WaveFlex biosensor based on S-tapered and waist-expanded technique for detection of glycosylated hemoglobin

Guiwei Zhang, Ragini Singh, Bingyuan Zhang, Santosh Kumar, and Guoru Li
Biomed. Opt. Express 14(11) 6100-6113 (2023)

LSPR-based cholesterol biosensor using a tapered optical fiber structure

Santosh Kumar, Brajesh Kumar Kaushik, Ragini Singh, Nan-Kuang Chen, Qing Shan Yang, Xia Zhang, Wenjun Wang, and Bingyuan Zhang
Biomed. Opt. Express 10(5) 2150-2160 (2019)

Taper-in-taper fiber structure-based LSPR sensor for alanine aminotransferase detection

Zhi Wang, Ragini Singh, Carlos Marques, Rajan Jha, Bingyuan Zhang, and Santosh Kumar
Opt. Express 29(26) 43793-43810 (2021)

Convex fiber-tapered seven core fiber-convex fiber (CTC) structure-based biosensor for creatinine detection in aquaculture

Muyang Li, Ragini Singh, Maria Simone Soares, Carlos Marques, Bingyuan Zhang, and Santosh Kumar
Opt. Express 30(8) 13898-13914 (2022)