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Improving the resolution of two-photon microscopy using pixel reassignment

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Abstract

An imaging system combining parallel detection and two-photon excitation is presented in this paper. In the proposed method, seven avalanche photodiodes (APD) are used in the detection path. The light is guided onto the APD sensor by a fiber bundle with seven fibers. The circular arranged fiber end faces together function as a detection array. The outer contour fiber ends deviate from optical axis center and thus obtain higher frequency information. Seven images are acquired simultaneously by the detection array. By using deconvolution and pixel reassignment to process the images acquired, both the resolution and the contrast of the final image are enhanced. The capability of image quality enhancement of the system constructed is demonstrated successfully both by theoretical simulation and experimental results.

© 2018 Optical Society of America

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