Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Depth-resolved velocimetry of Hagen–Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We quantitatively investigate the axial imaging properties of dynamic phase-contrast microscopy, with a special focus on typical combinations of tracer particles and magnifications that are used for velocimetry analysis. We show, for the first time, that a dynamic phase-contrast microscope, which is the integration of an all-optical novelty filter in a commercially available inverted microscope, can visualize three- dimensional velocity fields with a significantly reduced optical sectioning depth. The depth of field for dynamic phase-contrast microscopy is extracted from the three-dimensional response function and compared with the respective values for incoherent bright-field illumination. These results are then used to perform a depth-resolved particle image velocimetry analysis of Hagen–Poiseuille as well as electro-osmotically actuated flows in a microchannel.

© 2010 Optical Society of America

Full Article  |  PDF Article
More Like This
Vector tomographic X-ray phase contrast velocimetry utilizing dynamic blood speckle

Sarah C. Irvine, David M. Paganin, R. Aidan Jamison, Stephen Dubsky, and Andreas Fouras
Opt. Express 18(3) 2368-2379 (2010)

Laser Doppler field sensor for high resolution flow velocity imaging without camera

Andreas Voigt, Christian Bayer, Katsuaki Shirai, Lars Büttner, and Jürgen Czarske
Appl. Opt. 47(27) 5028-5040 (2008)

Blood cell assisted in vivo Particle Image Velocimetry using the confocal laser scanning microscope

Sung Min Choi, Wi Han Kim, Daniel Côté, Cheol-Woo Park, and Ho Lee
Opt. Express 19(5) 4357-4368 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved