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Simple spatial phase modulator for focal modulation microscopy

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Abstract

Focal modulation microscopy (FMM) is an emerging microscopy technique for fluorescence imaging of thick biological tissue in vivo. A spatial phase modulator is a critical component whose characteristics have a significant impact on the performance of a FMM system. We have designed a simple spatial phase modulator based on a tilting glass plate that provides superb modulation stability. Image quality has been improved remarkably after integrating such a modulator into a FMM system.

© 2009 Optical Society of America

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