Abstract

We present a new concept for fluorescence lifetime imaging (FLIM) based on time-resolved Hadamard imaging (HI). HI allows image acquisition by use of one single-point detector without requiring a moving scanning stage. Moreover, it reduces the influence of detector noise compared with raster scanning. By use of Monte Carlo simulations it could be confirmed that Hadamard transformation may decrease the error in lifetime estimation and in general in fluorescence parameter estimation when the signal-to-noise ratio is low and detector dark noise is high. This concept may find applications whenever the performance of FLIM or similar methods is limited by high dark-count rates and when the use of a single-point detector is preferable.

© 2005 Optical Society of America

Hadamard-transform fluorescence-lifetime imaging

Takahiko Mizuno and Tetsuo Iwata
Opt. Express 24(8) 8202-8213 (2016)

Time-resolved fluorescence imaging of solvent interactions in microfluidic devices

Richard K. P. Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark A. A. Neil, Andrew J. deMello, and Paul M. W. French
Opt. Express 13(16) 6275-6285 (2005)

Fluorescence lifetime tracking and imaging of single moving particles assisted by a low-photon-count analysis algorithm

Pengfa Chen, Qin Kang, JingJing Niu, YingYing Jing, Xiao Zhang, Bin Yu, Junle Qu, and Danying Lin
Biomed. Opt. Express 14(4) 1718-1731 (2023)

Performance and limitation estimation of a three-tap gated imaging sensor in wide field time-gated fluorescence lifetime imaging systems

Yun-Tzu Chang, Edward Van Sieleghem, Jiwon Lee, Pol Van Dorpe, and Chris Van Hoof
Appl. Opt. 60(24) 7446-7454 (2021)

Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

Sebastian Isbaner, Narain Karedla, Daja Ruhlandt, Simon Christoph Stein, Anna Chizhik, Ingo Gregor, and Jörg Enderlein
Opt. Express 24(9) 9429-9445 (2016)