In the past years, the use of laser based techniques for printing biomolecules has received significant attention as efficient techniques for direct and controllable bio-modification of solid surfaces and sensing applications. Update, laser printing has been applied for the fabrication of several types of biosensors with high spatial resolution including photosynthetic, enzymatic, and DNAzyme biosensors, targeting the detection of hazardous contaminants in food products and water. In this work, we present, a novel approach combining the Laser Induced Forward Transfer (LIFT) technique with novel laser photoinduced reactions from the area of ‘click chemistry’, for the creation of micropatterns of aptamers, specifically designed to bind the mycotoxin Ochratoxin A (OTA), on silicon nitride surfaces. In specific, it is shown that the LIFT technique may be used as direct technique for printing and photoactivating, in a single step process, thiol-modified aptamers, onto alkene- and alkyne-terminated surfaces. Light-mediated thiol-ene (or -yne) reactions effectively combine the classical benefits of click reactions with the advantages of a photo-initiated process, which can be activated at specific times and locations. In combination with the unique properties of the direct laser printing technique LIFT, this approach results in a powerful method for site-specific covalent immobilization of biomaterials onto sensor surfaces.
© 2017 Optical Society of AmericaPDF Article