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Conferences > OTS > 2016 > OTh4C > OTh4C.7

Visualizing Cellular Metabolic Processes With Combined Nonlinear Optical Microscopy

Alba Alfonso-Garcia, Tim Smith, Rupsa Datta, Enrico Gratton, Eric O. Potma, and Wendy Liu

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Abstract

Macrophages are key players in inflammatory processes. We combine label-free nonlinear optical microscopy techniques, namely coherent Raman scattering and fluorescence lifetime imaging, to link lipid and energy metabolism with macrophage phenotype in living cells.

© 2016 Optical Society of America

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