Probing relatively short molecules, such as the protein elastin (~200 nm), with optical tweezers requires manipulating trapped polystyrene beads at separations small compared to their micron dimensions. We discuss experimental complications arising from this proximity, such as hydrodynamic and optical interactions, and our efforts to minimize them.

© 2009 Optical Society of America

PDF Article


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription