Bleaching-Assisted Multichannel Microscopy

Antony Orth; Timothy Doughney; Philipp Reineck; Hannah Brown; Jeremy Thompson; Brant C. Gibson

doi:10.1364/OMP.2017.OmW2D.3

Optics in the Life Sciences Congress
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper OmW2D.3
•https://doi.org/10.1364/OMP.2017.OmW2D.3

Bleaching-Assisted Multichannel Microscopy

Antony Orth, Timothy Doughney, Philipp Reineck, Hannah Brown, Jeremy Thompson, and Brant C. Gibson

Optical Molecular Probes, Imaging and Drug Delivery 2017

San Diego, California United States
2–5 April 2017
ISBN: 978-1-943580-25-5

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Probes and Techniques for Tissue Characterization II (OmW2D)

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A. Orth, T. Doughney, P. Reineck, H. Brown, J. Thompson, and B. C. Gibson, "Bleaching-Assisted Multichannel Microscopy," in Optics in the Life Sciences Congress, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper OmW2D.3.

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Abstract

We show that photobleaching can be harnessed as valuable dimension of contrast for fluorescence microscopy. Our photostability-based image unmixing technique more than doubles the multiplexing capability of any multichannel fluorescence microscope.

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