Definite diagnosis of bile duct malignancy is based on duct stricture biopsy. Currently, peroral cholangioscopy is the only technology available for guiding stricture biopsy. Unfortunately, the sensitivity of cholangioscopy-guided bile duct biopsy can be as low as 30%. Siebel et al. has developed an ultra thin (1.2-1.6 mm diameter), ultra flexible (6 mm bending radius) scanning fiber endoscope (SFE) that allows video-rate (30 Hz) high-resolution (600 lines) true color imaging (Fig. 1.A-C) [1]. Time-resolved fluorescence spectroscopy (TRFS) allows assessing tissue biochemical composition, an important biomarker of epithelial cancer [2]. We hypothesize that the combination of high-resolution SFE imaging with TRFS biochemical assessment should improve the sensitivity of bile duct biopsy. Here, we present the first TRFS data ever collected from bile ducts, demonstrating the feasibility of measuring bile duct endogenous TRFS signal with adequate SNR upon acceptable power levels of UV light excitation.

© 2011 OSA

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