High speed imaging of calcium dynamics in cardiomyocytes with a flexible light-sheet fluorescence microscope

Liuba Dvinskikh; Liuba Dvinskikh; Hugh Sparks; Julia Gorelik; Sian Harding; Ken MacLeod; Chris Dunsby; Chris Dunsby

doi:10.1364/NTM.2021.NM2C.6

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NM2C.6
•https://doi.org/10.1364/NTM.2021.NM2C.6

High speed imaging of calcium dynamics in cardiomyocytes with a flexible light-sheet fluorescence microscope

Liuba Dvinskikh, Hugh Sparks, Julia Gorelik, Sian Harding, Ken MacLeod, and Chris Dunsby

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Light Sheet and Ultra-violet Microscopies (NM2C)

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L. Dvinskikh, H. Sparks, J. Gorelik, S. Harding, K. MacLeod, and C. Dunsby, "High speed imaging of calcium dynamics in cardiomyocytes with a flexible light-sheet fluorescence microscope," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NM2C.6.

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Abstract

Flexible dual-channel inverted light sheet fluorescence microscopy enables high speed 2D imaging and quantitative analysis of calcium sparks and transients in cardiomyocytes and 3D remote refocused imaging of calcium dynamics in stem cell derived cardiomyocytes.

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