Dual-view oblique plane microscopy

Hugh Sparks; Jorge Almagro; Axel Behrens; Guillaume Salbreux; Chris Dunsby; Chris Dunsby

doi:10.1364/NTM.2021.NM2C.5

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NM2C.5
•https://doi.org/10.1364/NTM.2021.NM2C.5

Dual-view oblique plane microscopy

Hugh Sparks, Jorge Almagro, Axel Behrens, Guillaume Salbreux, and Chris Dunsby

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Light Sheet and Ultra-violet Microscopies (NM2C)

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H. Sparks, J. Almagro, A. Behrens, G. Salbreux, and C. Dunsby, "Dual-view oblique plane microscopy," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NM2C.5.

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Abstract

Dual-view Oblique Plane Microscopy (dOPM) enables single-objective multi-view light-sheet fluorescence microscopy. This talk introduces the dOPM concept and demonstrates optical resolution performance with exemplar 3D datasets of fluorescence bead samples and fixed multicellular spheroids.

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