Adaptive Optics Light-Sheet Microscopy For Functional Neuroimaging

Antoine Hubert; Antoine Hubert; Fabrice Harms; Sophia Imperato; Vincent Loriette; Cynthia Veilly; Xavier Levecq; Georges Farkouh; François Rouyer; Alexandra Fragola

doi:10.1364/NTM.2021.NM2C.4

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NM2C.4
•https://doi.org/10.1364/NTM.2021.NM2C.4

Adaptive Optics Light-Sheet Microscopy For Functional Neuroimaging

Antoine Hubert, Fabrice Harms, Sophia Imperato, Vincent Loriette, Cynthia Veilly, Xavier Levecq, Georges Farkouh, François Rouyer, and Alexandra Fragola

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Light Sheet and Ultra-violet Microscopies (NM2C)

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A. Hubert, F. Harms, S. Imperato, V. Loriette, C. Veilly, X. Levecq, G. Farkouh, F. Rouyer, and A. Fragola, "Adaptive Optics Light-Sheet Microscopy For Functional Neuroimaging," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NM2C.4.

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Abstract

We present a novel implementation of extended-scene adaptive optics for light-sheet microscopy based on direct wavefront sensing for fast aberration correction. We report AO-enhanced functional images of GCaMP neurons in the live drosophila brain.

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