Multiplexed PSF engineering - photon-efficient simultaneous multicolor 3D particle tracking

Nadav Opatovski; Yael Shalev Ezra; Lucien E. Weiss; Boris Ferdman; Reut Orange; Yoav Shechtman; Yoav Shechtman

doi:10.1364/NTM.2021.NF1C.3

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NF1C.3
•https://doi.org/10.1364/NTM.2021.NF1C.3

Multiplexed PSF engineering - photon-efficient simultaneous multicolor 3D particle tracking

Nadav Opatovski, Yael Shalev Ezra, Lucien E. Weiss, Boris Ferdman, Reut Orange, and Yoav Shechtman

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Super-Resolution II (NF1C)

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N. Opatovski, Y. S. Ezra, L. E. Weiss, B. Ferdman, R. Orange, and Y. Shechtman, "Multiplexed PSF engineering - photon-efficient simultaneous multicolor 3D particle tracking," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NF1C.3.

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Abstract

We demonstrate photon-efficient, multicolor, large FOV 3D localization microscopy with high spatiotemporal resolution by multiplexing spectrally-defined, single-channel PSFs. This is accomplished by encoding spectral and 3D information into the PSFs.

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