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Conferences > NTM > 2017 > NS1C > NS1C.2

3D imaging from 2D scanning with helical point spread function engineering

Clemens Roider, Rainer Heintzmann, Rafael Piestun, and Alexander Jesacher

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Abstract

Scanning microscopes with pixelated detectors combine high spatial resolution and light collection efficiency. We record and reconstruct 3D sample information with only a 2D scan by suitably modifying the point spread function of the microscope. We introduce a data post processing scheme for producing 3D images from the collected raw data.

© 2017 Optical Society of America

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