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Conferences > NTM > 2013 > NTh2B > NTh2B.1

Frequency Multiplexed in vivo Multiphoton Phosphorescence Lifetime Microscopy

Scott S. Howard, Adam Straub, Nicholas G. Horton, Demirhan Kobat, Aamir Khan, Eric DeLeon, Kenneth R. Olson, and Chris Xu

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Abstract

Multiphoton Microscopy and Phosphorescence Lifetime Microscopy are combined to produce full-frame lifetime resolved 3D images in vivo. Parallel excitation and acquisition of two-photon emission is achieved via a frequency multiplexed multi-foci excitation beam.

© 2013 Optical Society of America

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