Ratiometric 3D scanning cytometer for quantifying cell-surface biomarker expression within intact tissues

Jonathan T.C. Liu; Jonathan T.C. Liu; Michael J. Mandella; Mike W. Helms; James M. Crawford; Christopher H. Contag; Gordon S. Kino

doi:10.1364/NTM.2009.NWC2

Advances in Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2009),
paper NWC2
•https://doi.org/10.1364/NTM.2009.NWC2

Ratiometric 3D scanning cytometer for quantifying cell-surface biomarker expression within intact tissues

Jonathan T.C. Liu, Michael J. Mandella, Mike W. Helms, James M. Crawford, Christopher H. Contag, and Gordon S. Kino

Novel Techniques in Microscopy 2009

Vancouver Canada
26–30 April 2009
ISBN: 978-1-55752-871-1

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Endomicroscopy (NWC)

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J. T. C. Liu, M. J. Mandella, M. W. Helms, J. M. Crawford, C. H. Contag, and G. S. Kino, "Ratiometric 3D scanning cytometer for quantifying cell-surface biomarker expression within intact tissues," in Advances in Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper NWC2.

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Abstract

There are unique challenges in image interpretation during in vivo three-dimensional microscopy due to irregular tissue staining and inefficient rinsing of unbound probes in thick tissues. We have developed a two-color ratiometric microscopy technique that not only reduces image contrast due to nonspecific probe accumulation, but also provides a quantitative 3D map of cellular biomarker expression. This strategy will enable unambiguous determination of disease states for real-time optical biopsy.

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