Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Optical diffraction tomography for high resolution live cell imaging

Not Accessible

Your library or personal account may give you access

Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

© 2009 Optical Society of America

PDF Article
More Like This
Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld
NTuA1 Novel Techniques in Microscopy (NTM) 2009

White-light Diffraction Tomography (WDT) of Live Cells

Taewoo Kim, Renjie Zhou, Lynford L. Goddard, and Gabriel Popescu
BM4B.6 Biomedical Optics (BIOMED) 2014

3-D Imaging of Malaria-infected Human Red Blood Cells Using Optical Diffraction Tomography

Kyoohyun Kim, HyeOk Yoon, and YongKeun Park
SM4P.2 CLEO: Science and Innovations (CLEO:S&I) 2014

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved