Multiphoton fluorescence microscopy for functional imaging of biomolecules

Ahmed A. Heikal; Watt W. Webb

doi:10.1364/NLO.2002.ThA4

Nonlinear Optics: Materials, Fundamentals and Applications
OSA Trends in Optics and Photonics (Optica Publishing Group, 2002),
paper ThA4
•https://doi.org/10.1364/NLO.2002.ThA4

Multiphoton fluorescence microscopy for functional imaging of biomolecules

Ahmed A. Heikal and Watt W. Webb

Nonlinear Optics: Materials, Fundamentals and Applications 2002

Wailea, Maui, Hawaii United States
29 July–2 August 2002
ISBN: 1-55752-721-0

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Bio-Chemical Nonlinear Optics (ThA)

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A. A. Heikal and W. W. Webb, "Multiphoton fluorescence microscopy for functional imaging of biomolecules," in Nonlinear Optics: Materials, Fundamentals and Applications, A. Sawchuk, ed., Vol. 79 of OSA Trends in Optics and Photonics (Optica Publishing Group, 2002), paper ThA4.

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Abstract

Molecular level understanding of numerous biological processes can be elucidated using multiphoton fluorescence spectroscopy, dynamics, and microscopy. We exploit the intrinsic biomolecular fluorescence in live cells for non-invasive and real-time monitoring of in vivo biological processes including the mitochondrial respiratory chain.

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