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Using Multi-focal Structure Illumination to Study Cell Structure

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Abstract

Multifocal structured illumination microscopy (MSIM) is an imaging technique that combines structure illumination microscopy (SIM) with laser confocal scanning microscopy (LCSM). It has the advantage of both techniques: two-fold increase in lateral resolution as SIM does and perfect optical section ability as LCSM does. Therefore, MSIM is suitable for observing fine structure in thick biological samples. Taking advantage of such characteristics, we have built up a super-resolution system including both hardware and software. It integrates the conventional SIM with novel MISM, and can be easily switched between different modes. Using this system, we did some preliminary bioresearch, such as observing the tubulin in Hela cells and the actin in thicker cells such as insulinoma INS-1 cells. We show a increase in optical resolution as observing both samples.

© 2013 Optical Society of America

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