Abstract

We present the technical development of a high-throughput super-resolution imaging system based on optimized stochastic optical reconstruction microscopy. This system enables robust and reliable super-resolution imaging of nanoscale chromatin folding over a large field of view on standard pathological tissue. Super-resolution microscopy revealed fragmented chromatin folding throughout all stages of carcinogenesis.

© 2020 The Author(s)

PDF Article  |   Presentation Video
More Like This
Imaging Nuclear Architecture at the Nanoscale for Cancer Detection and Prognosis

Yang Liu, Shikhar Uttam, Hoa V. Pham, Justin LaFace, and Douglas J. Hartman
OT1C.6 Bio-Optics: Design and Application (BODA) 2015

Nanoscale Imaging of Chromatin with Labeled and Label-Free Super-Resolution Microscopy and Partial-Wave Spectroscopy

Adam Eshein, Yue Li, Xiang Zhou, Graham Spicer, The-Quyen Nguyen, Luay M. Almassalha, John E. Chandler, Scott Gladstein, Biqin Dong, Cheng Sun, Hao F. Zhang, and Vadim Backman
MW2A.4 Microscopy Histopathology and Analytics (Microscopy) 2018

Three-dimensional nanoscale nuclear architecture mapping for improved cancer risk stratification

Shikhar Uttam and Yang Liu
11076_38 European Conference on Biomedical Optics (ECBO) 2019

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Presentation Video

Conference videos freely accessible until 30 June 2021 and available to subscribers only thereafter.

More Like This
Imaging Nuclear Architecture at the Nanoscale for Cancer Detection and Prognosis

Yang Liu, Shikhar Uttam, Hoa V. Pham, Justin LaFace, and Douglas J. Hartman
OT1C.6 Bio-Optics: Design and Application (BODA) 2015

Nanoscale Imaging of Chromatin with Labeled and Label-Free Super-Resolution Microscopy and Partial-Wave Spectroscopy

Adam Eshein, Yue Li, Xiang Zhou, Graham Spicer, The-Quyen Nguyen, Luay M. Almassalha, John E. Chandler, Scott Gladstein, Biqin Dong, Cheng Sun, Hao F. Zhang, and Vadim Backman
MW2A.4 Microscopy Histopathology and Analytics (Microscopy) 2018

Three-dimensional nanoscale nuclear architecture mapping for improved cancer risk stratification

Shikhar Uttam and Yang Liu
11076_38 European Conference on Biomedical Optics (ECBO) 2019