We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.

© 2020 The Author(s)

PDF Article
More Like This
Use of acceptor fluorescence for determining FRET lifetimes

Michael K. Y. Hughes, Simon M. Ameer–Beg, Marion Peter, and Tony Ng
5139_88 European Conference on Biomedical Optics (ECBO) 2003

Near Infrared FRET using Wide-field Fluorescence Lifetime Imaging in Live Animals

Lingling Zhao, Ken Abe, Margarida Barroso, and Xavier Intes
88010A European Conference on Biomedical Optics (ECBO) 2013

FRET detection for neurobiological applications using a Total Internal Reflection Fluorescence Lifetime Imaging Microscope

V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M.C. Potier, F. Druon, P. Georges, and S. Lévêque-Fort
7367_11 European Conference on Biomedical Optics (ECBO) 2009


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription