Abstract
We developed an AO approach to improving multiphoton microscopy in the highly distorting mouse long bone environment, which uses an SHG guidestar with SHWFS. Together with a high stroke DM, this is capable of correcting the high magnitude aberrations present in the curved sample, thereby improving the fluorescence intensity of the imaging PSF, revealing features of collagen distribution and the bone marrow environment.
© 2021 The Author(s)
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