The field of nonlinear optical (NLO) microscopy has enjoyed vibrant development over the past two decades. Advances in NLO microscopy offers more sensitive, deeper and faster imaging. More importantly, NLO has grown from its root in fluorescence to a broad range of contrast mechanisms. These technological advances have broad impact on biology and medicine. Using the area of neurobiology as an example, this presentation seeks to highlight key technical advances and their biomedical impact. Article not available.

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