Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Laser Applications to Chemical and Environmental Analysis
  • Technical Digest (Optica Publishing Group, 2004),
  • paper TuC3

Temporal- and spectral analysis of multi-color FLIM measurements

Not Accessible

Your library or personal account may give you access

Abstract

Spectral unmixing procedures have been established to improve the image quality of multi-stained fluorescence samples. Based on the known spectral shape of different chromophores their relative contributions to the overall fluorescence emission can be derived. Thus a separation of sub-structures containing chromophores with overlapping emission spectra becomes possible if a multi-detector assembly is used. However, energy tansfer processes between emitting species can influence the results significantly. In this work we will present an approach which uses both time- and spectral information to discriminate dynamic processes from simple concentration effects in fluorescence imaging measurements. Our instrumentation covers a combination of a TCSPC based multi-wavelength detection technique together with a laser scanning microscope. The general solution obtained for two emitters exchanging energy by excited state transfer will be discussed.

© 2004 Optical Society of America

PDF Article
More Like This
Fluorescence Lifetime Imaging by Multi-Detector TCSPC

Wolfgang Becker, Axel Bergmann, Giovanni Biscotti, and Christoph Biskup
WD1 Biomedical Topical Meeting (BIOMED) 2004

Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling

Sumeet Rohilla, Benedikt Krämer, Felix Koberling, Ingo Gregor, and Andreas C. Hocke
11076_45 European Conference on Biomedical Optics (ECBO) 2019

Time-and Wavelength-Resolved Autofluorescence Detection by Multi-Dimensional TCSPC

Wolfgang Becker, Axel Bergmann, Dietrich Schweitzer, and Martin Hammer
ThE2 European Conference on Biomedical Optics (ECBO) 2005

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved