Abstract

Phage display, an attracting attention, and recipient of 2018 Nobel Prize in Chemistry is a method for screening functional bacteriophages (phage) with high binding affinity for the target molecules, can enhance selectivity and sensitivity when applied to sensors [1]. In particular, the functional phage is expected to be suitable for the SERS plat-form because low selectivity is a problem even though the SERS platform is known to have high sensitivity. Here we present a method for applying functional phage to the SERS platform as a bio-receptor [2]. The binding affinity of the functional phage for the target substance was verified by the isotherm titration calorimetry (ITC). As a result, it was confirmed that the selectivity and sensitivity of the SERS platform into which the phage was introduced were enhanced in proportion to the binding affinity.

© 2019 Japan Society of Applied Physics, The Optical Society (OSA)