Abstract
Two-photon excitation microscopy is widely used to observe interiors of biological tissues with utilizing the long penetration and the depth discrimination capability. However, the use of near-infrared light decreases the spatial resolution compared to that in typical fluorescence microscopes using visible light for excitation. The wave distortion and scattering also degrade the spatial resolution in tissue. Although several super-resolution techniques are utilized for two-photon excitation microscopy, improving the spatial resolution in the deep imaging still remains as a challenge.
© 2017 Japan Society of Applied Physics, Optical Society of America
PDF ArticleMore Like This
Hang Yu, P. Thilanka Galwaduge, Venkatakaushik Voleti, Kripa B. Patel, Wenze Li, Mohammed A. Shaik, and Elizabeth M.C. Hillman
NW4C.3 Novel Techniques in Microscopy (NTM) 2017
Guangyuan Zhao, Cuifang Kuang, Cheng Zheng, and Xu Liu
JT3A.60 3D Image Acquisition and Display: Technology, Perception and Applications (3D) 2016
Yasunori Nawa, Yasuo Yonemaru, Atsushi Kasai, Nicholas I. Smith, Hitoshi Hashimoto, Satoshi Kawata, and Katsumasa Fujita
15a_C31_7 JSAP-OSA Joint Symposia (JSAP) 2016