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Diagram Method for Resolution Limit Calculation in Laser Microscopy

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Abstract

In 1873, Ernst Abbe established the modem theory of image formation in optical microscopy and derived the well-known formula for optical resolution, d=λ/2NA, which corresponds to frequency cutoff (resolution limit) of 2NA/λ. Recently many kinds of laser microscopy have been developed by means of a variety of light-matter interactions. Although the Abbe’s definition of resolution limit is still used as the standard, it is relatively unknown that 2NA/λ can be applied only to the microscopy utilizing χ(1)-derived light-matter interaction such as linear absorption. If taking advantage of higher order χ(1)-derived interactions (i≧2), the resolution limit may surpass 2NA/λ. In this study, we formulated the rules for the calculation of resolution limit in all laser microscopy that employ arbitrary interactions.

© 2017 Japan Society of Applied Physics, Optical Society of America

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