Super-resolution microscopy opens up a new era for visualizing the dynamic subcellular structure and interaction in a whole new scale[1]. The methods to achieve super-resolution can be largely divided into two categories: (1) targeted modulation; and (2) stochastic modulation/fluctuation analysis. In stochastic single-molecule localization microscopy, the spatial resolution is gained through the scarification of temporal resolution, to ensure the sufficient sampling of the specimen. However, in super-resolution microscopy, the high spatial resolution is generally at a price of the imaging speed[2]. Super-resolution optical fluctuation imaging (SOFI) is a new technique which can allow multiple emitters within one diffraction limited region to be at on state simultaneously, and using the fluctuation dynamics to achieve super-resolution. Thus, it provides high spatial and temporal resolution simultaneously.

© 2015 Japan Society of Applied Physics, Optical Society of America

PDF Article
More Like This
Live cell high spatiotemporal stochastic optical fluctuation imaging (SOFI) nanoscopy

Xuanze Chen, Zhiping Zeng, Xi Zhang, Pingyong Xu, Yujie Sun, and Peng Xi
JTh5A.1 CLEO: Applications and Technology (CLEO_AT) 2015

Sparsity-based super-resolution optical fluctuation imaging

Oren Solomon, Maor Mutzafi, Xiyu Yi, Shimon Weiss, Yonina C. Eldar, and Mordechai Segev
AM4O.4 CLEO: Applications and Technology (CLEO_AT) 2016

Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

Adrian Makowski, Gur Lubin, Ron Tenne, Aleksandra Sroda, Uri Rossman, Dan Oron, and Radek Lapkiewicz
FW7E.6 Frontiers in Optics (FiO) 2020


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription