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Large Area Raman Spectroscopic Imaging of Unstained Mouse Brain Slice with Sub-micron Spatial Resolution

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Abstract

Recently, large area Raman imaging of unstained mouse brain tissues is reported using coherent Raman microscopies featuring high image speed acquisitions resulting from the enhanced Raman signals [1-3]. However, this increase in imaging speed comes at the expense of losing full spectral information. Coherent Raman microscopy typically acquires an image at a single band or a few 100 cm−1 in the case of multiplex versions. Here, we report large area Raman spectroscopic imaging of mouse brain slice using a slit-scanning Raman microscope capable of obtaining large images with full spectral information while maintaining sub-micron spatial resolution

© 2014 Japan Society of Applied Physics, Optical Society of America

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