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Fluorescent Nano Diamonds for High Spatial Resolution Live Cell Imaging by Direct Electron Beam Excitation

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Abstract

Nano bio-imaging is highly desirable to understand the cellular functions, because these functions are emerged from localization and the dynamic interactions of the cellular molecules in biological cells. Fluorescence microscopes are widely used to follow the processes and dynamics of specific cellular components within living cells. Optical signals include a lot of valuable information about specimens, such as intensity, wavelength and fluorescence life time. However, the spatial resolution is limited by the diffraction to about half the wavelength of the light used.

© 2013 Japan Society of Applied Physics, Optical Society of America

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