3D fluorescent microscopy by multiplexed phase-space measurements

Hsiou-Yuan Liu; Jingshan Zhong; Laura Waller

doi:10.1364/ISA.2017.ITh4E.3

Imaging and Applied Optics 2017 (3D, AIO, COSI, IS, MATH, pcAOP)
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper ITh4E.3
•https://doi.org/10.1364/ISA.2017.ITh4E.3

3D fluorescent microscopy by multiplexed phase-space measurements

Hsiou-Yuan Liu, Jingshan Zhong, and Laura Waller

Imaging Systems and Applications 2017

San Francisco, California United States
26–29 June 2017
ISBN: 978-1-943580-29-3

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Spectral Image Processing (ITh4E)

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H. Liu, J. Zhong, and L. Waller, "3D fluorescent microscopy by multiplexed phase-space measurements," in Imaging and Applied Optics 2017 (3D, AIO, COSI, IS, MATH, pcAOP), OSA Technical Digest (online) (Optica Publishing Group, 2017), paper ITh4E.3.

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Abstract

We present a multi-shot coded aperture 3D fluorescent imaging method based on multiplexed phase-space sampling theory. Our reconstructed volumes achieve diffraction-limited resolution from only 100 raw images, by exploiting the sparse nature of the sample.

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