A method is proposed for estimating the bispectral characteristics of fluorescent objects by using two illuminant projections. The Donaldson matrix represents the bispectral radiance factor consisting of two components: a reflected radiance factor and a luminescent radiance factor. We describe the spectral characteristics of the observed matrix and model the matrix. An algorithm is presented to estimate the entire elements of the Donaldson matrix based on only two sets of spectral sensor outputs under two different illuminants. The feasibility of the proposed method is confirmed in experiments using a variety of fluorescent samples.

© 2015 Optical Society of America

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