HiLo-Regularized Digital Light Sheet Microscopy for live 3D imaging of developing embryos and live animals

George Barbastathis; George Barbastathis; Dipanjan Bhattacharya; Dipanjan Bhattacharya; Vijay Raj Singh; Chen Zhi; Chen Zhi; Peter T. C. So; Peter T. C. So; Paul Matsudaira

doi:10.1364/ISA.2013.IM4E.2

Imaging and Applied Optics
OSA Technical Digest (online) (Optica Publishing Group, 2013),
paper IM4E.2
•https://doi.org/10.1364/ISA.2013.IM4E.2

HiLo-Regularized Digital Light Sheet Microscopy for live 3D imaging of developing embryos and live animals

George Barbastathis, Dipanjan Bhattacharya, Vijay Raj Singh, Chen Zhi, Peter T. C. So, and Paul Matsudaira

Imaging Systems and Applications 2013

Arlington, Virginia United States
23–27 June 2013
ISBN: 978-1-55752-975-6

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Biomedical Applications (IM4E)

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G. Barbastathis, D. Bhattacharya, V. R. Singh, C. Zhi, P. T. C. So, and P. Matsudaira, "HiLo-Regularized Digital Light Sheet Microscopy for live 3D imaging of developing embryos and live animals," in Imaging and Applied Optics, OSA Technical Digest (online) (Optica Publishing Group, 2013), paper IM4E.2.

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Abstract

We describe an approach to obtain three-dimensional (3D) structured illumination in digital light sheet microscopy, and present experimental proof of contrast improvements.

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