Abstract
A ultrashort pulse laser microbeam system has been constructed for microsurgery on cell. The relations of laser wavelength, pulse duration and pulse energy to punching effects and self-healing process were studied. The experimental results demonstrate that picosecond pulse laser microbeam offers many advantages in cell microsurgery. The mechanism of punching by picosecond microbeam is high field puncture instead of heat effect, and is irrelevant to cell kinds and colors. The diameter and depth of microsurgery can therefore be easily controlled by adjusting the laser pulse energy.
© 1996 Optical Society of America
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