Abstract

Bleeding disorder related to platelet number and functionality is a key issue before conducting surgery and monitoring the patient’s condition; therefore, reduction in the platelet number or disorder in their function cause bleeding risk. In this study, we introduce an optical evaluation method of platelet aggregation within a channel in a microfluidic chip by measuring the laser speckle size, which is related to the retarded blood flow in the microfluidic channel. With a whole blood sample, the speckle size was 28.54 ± 0.72 µm, and this is due to the aggregation of platelets. On the other hand, after removing the platelets from the blood, we found that the speckle size was significantly reduced to 27.04 ± 1.23 um. Also, by adding agonist containing ADP that can activate the platelets, the difference in speckle size was further increased to 32.89 ± 1.69 um. These findings may provide a simple optical technique with microfluidics to test the function of platelets in the diagnosis of bleeding disorders and may help monitor anti-platelet therapies.

© 2020 The Author(s)

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