Abstract

We constructed a hyperspectral multiphoton microscope that records 48 channels of excitation/emission spectral data per pixel, while remaining insensitive to optical scattering. We demonstrate imaging of multiple spectrally overlapping fluorophores in mouse cortex, in vivo.

© 2015 Optical Society of America

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More Like This
Hyperspectral Imaging in Live Mouse Cortex Using a 48-Channel Multiphoton Microscope

A. J. Bares, M. A. Pender, M. A. Mejooli, S. Tilley, K. E. Chen, J. Dong, P. C. Doerschuk, and C. B. Schaffer
FTh5D.5 Frontiers in Optics (FiO) 2016

Hyperspectral multiphoton microscopy for in vivo visualization of spectrally-overlapped fluorescent labels

Menansili A. Mejooli, Amanda J. Bares, Scott Leddon, Steven Tilley, Jingyuan Dong, Minsoo Kim, Deborah J. Fowell, Nozomi Nishimura, and Chris B. Schaffer
NW1C.3 Novel Techniques in Microscopy (NTM) 2019

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Michael J. Levene and Thomas H. Chia
NMD2 Novel Techniques in Microscopy (NTM) 2009