In optical microscopy, the depth of field (DOF) is limited by the physical characteristics of the image forming systems. Take a picture with all the targeted regions becomes impossible and we must resort to techniques of digital image processing. In order to extend the DOF of a microscopic system, this paper a method of image fusion and multi-pronged approach based module gradient color planes is proposed. Experimental results are presented using metallic samples. The proposed method is simple, fast and free of artifacts or false color.

© 2015 Optical Society of America

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