Abstract

The quadrated pupil and double-helix PSF are modifications of epifluorescence microscopes that enable precise measurements of the single-molecule orientation. We explore how position estimation beyond the diffraction limit is affected by single-molecule orientation within cells.

© 2013 Optical Society of America

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More Like This
Measuring the 3D Position and Orientation of Single Molecules Simultaneously and Accurately with the Double Helix Microscope

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CTu3N.3 CLEO: Science and Innovations (CLEO_SI) 2013

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